phosphomtor ser2448 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc phosphomtor ser2448
Phosphomtor Ser2448, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4763 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phosphomtor+ser2448/Phospho-mTOR+(Ser2448)+Antibody/pm41223896-283-71-74
Average 97 stars, based on 4763 article reviews

phosphomtor ser2448 - by Bioz Stars, 2026-09

97/100 stars

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Article Title: Sex differences in the modulation of anxiety- and depression-like behaviors by matrix metalloproteinase-9 expression levels in mice
Article Snippet: 12 ANTIBODY DILUTION REFERENCE SUPPLIER RRIDs REFERENCES β-tubulin III (rb, m) 1:20000 T2200/T8660 Sigma‒ Aldrich AB_262133/ AB_477590 [1], [2], [3], [4], [5], [6] BDNF (rb) 1:500 ab108319 Abcam AB_10862052 [7], [8], [9], [10], [11] mTOR (m) 1:1000 #4517 Cell Signaling Technology AB_1904056 [12], [13], [14], [15] PhosphomTOR (Ser2448) (rb) 1:1000 #2971 Cell Signaling Technology AB_330970 [12], [13], [16], [17], [18], [19] PSD95 (g) 1:200 sc-8575 Santa Cruz Biotechnology AB_2092357 [18], [20], [21] Synapsin Ia/b (m) 1:200 sc-390867 Santa Cruz Biotechnology AB_3678685 [22], [23] 13 14 15 Figure S1.

Article Title: Sex differences in the modulation of anxiety- and depression-like behaviors by matrix metalloproteinase-9 expression levels in mice
Article Snippet: ANTIBODY DILUTION REFERENCE SUPPLIER RRIDs REFERENCES β-tubulin III (rb, m) 1:20000 T2200/T8660 Sigma‒ Aldrich AB_262133/ AB_477590 [1], [2], [3], [4], [5], [6] BDNF (rb) 1:500 ab108319 Abcam AB_10862052 [7], [8], [9], [10], [11] mTOR (m) 1:1000 #4517 Cell Signaling Technology AB_1904056 [12], [13], [14], [15] PhosphomTOR (Ser2448) (rb) 1:1000 #2971 Cell Signaling Technology AB_330970 [12], [13], [16], [17], [18], [19]

Blocking Assay:

Article Title: Integrative Multi-omics and Network Pharmacology Reveal the Mechanisms of Xiaoyaosan in Treating Depression
Article Snippet: .. Then, transfer to PVDF membranes was conducted at 200 mA for 90 min, and 5% ACS Omega http://pubs.acs.org/journal/acsodf Article https://doi.org/10.1021/acsomega.4c10769 ACS Omega 2025, 10, 24140−24159 24142 skim milk was added as a blocking agent at room temperature for 2 h prior to overnight incubation (4 °C) with the following primary antibodies: AKT (1:1000, CST), FOXP3 (1:1000, Abcam), mTOR (1:1000, CST), phospho-AKT (Ser473) (1:1000, CST), PIK3CA (1:1000, Invitrogen), phosphomTOR (Ser2448) (1:1000, CST), phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) (1:1000, Invitrogen), and ROR gamma T (1:1000, Invitrogen). .. After three washes in TBST, the membranes were incubated with suitable IgG-HRP secondary antibodies (1:3000, Bioss) at room temperature for 1 h followed by TBST washing and ECL development.

Article Title: Integrative Multi-omics and Network Pharmacology Reveal the Mechanisms of Xiaoyaosan in Treating Depression
Article Snippet: .. Then, transfer to PVDF membranes was conducted at 200 mA for 90 min, and 5% https://doi.org/10.1021/acsomega.4c10769 ACS Omega 2025, 10, 24140−24159 24142 skim milk was added as a blocking agent at room temperature for 2 h prior to overnight incubation (4 °C) with the following primary antibodies: AKT (1:1000, CST), FOXP3 (1:1000, Abcam), mTOR (1:1000, CST), phospho-AKT (Ser473) (1:1000, CST), PIK3CA (1:1000, Invitrogen), phosphomTOR (Ser2448) (1:1000, CST), phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) (1:1000, Invitrogen), and ROR gamma T (1:1000, Invitrogen). .. After three washes in TBST, the membranes were incubated with suitable IgG-HRP secondary antibodies (1:3000, Bioss) at room temperature for 1 h followed by TBST washing and ECL development.

Incubation:

Article Title: Glutathione peroxidase (GPX1) - Selenocysteine metabolism preserves the follicular fluid's (FF) redox homeostasis via IGF-1- NMD cascade in follicular ovarian cysts (FOCs).
Article Snippet: Follicular ovarian cysts (FOCs) are characterized by follicles in the ovaries that are >20 mm in diameter and persist for >10 days without the corpus luteum, leading to anovulation, dysregulation of folliculogenesis and subfertility in humans and livestock species.. Despite their clinical significance, the precise impact of FOCs on oocyte reserve, maturation, and quality still needs to be explored.. While FOCs are observed in both human and livestock populations, they are notably prevalent in livestock species.

Article Title: Integrative Multi-omics and Network Pharmacology Reveal the Mechanisms of Xiaoyaosan in Treating Depression
Article Snippet: .. Then, transfer to PVDF membranes was conducted at 200 mA for 90 min, and 5% https://doi.org/10.1021/acsomega.4c10769 ACS Omega 2025, 10, 24140−24159 24142 skim milk was added as a blocking agent at room temperature for 2 h prior to overnight incubation (4 °C) with the following primary antibodies: AKT (1:1000, CST), FOXP3 (1:1000, Abcam), mTOR (1:1000, CST), phospho-AKT (Ser473) (1:1000, CST), PIK3CA (1:1000, Invitrogen), phosphomTOR (Ser2448) (1:1000, CST), phospho-PI3 kinase p85 alpha + gamma (Tyr467 + Tyr199) (1:1000, Invitrogen), and ROR gamma T (1:1000, Invitrogen). .. After three washes in TBST, the membranes were incubated with suitable IgG-HRP secondary antibodies (1:3000, Bioss) at room temperature for 1 h followed by TBST washing and ECL development.

Membrane:

Western Blot:

Article Title: Staufen2 dysregulation in neurodegenerative disease.
Article Snippet: .. Antibodies used for Western blotting The antibodies used for Western blotting and their dilutions were as follows: mouse anti-ATXN2 antibody (Clone 22/ ATXN2) (1:4000 dilution, BD Biosciences, catalog no.: 611378), LC3B Antibody (1:7000 dilution, Novus Biologicals, catalog no.: NB100-2220), SQSTM1/p62 antibody (1:4000 dilution, Cell Signaling, catalog no.: 5114), mTOR antibody (1:4000 dilution, Cell Signaling, catalog no.: 2972), PhosphomTOR (Ser2448) antibody (1:3000 dilution, Cell Signaling, catalog no.: 2971), GAPDH (14C10) rabbit mAb (1:5000 dilution, Cell Signaling, catalog no.: 2118), GFP (D5.1) rabbit mAb (1:5000 dilution, Cell Signaling, catalog no.: 2956), and monoclonal anti-b-actin-peroxidase antibody (clone AC-15) (1:30,000 dilution, Sigma–Aldrich, catalog no.: A3854). ..

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